Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Construction of repeat-free fluorescence in situ hybridization probes
FISH probes are generally made out of BAC clones with genomic DNA containing a variable amount of repetitive DNA that will need to be removed or blocked for FISH analysis. To generate repeat free (RF) Probes without loss in genomic coverage, a random library is made from BAC clones by whole-genome amplification (WGA). Libraries are denatured in the presence of excess C(0)t-1 DNA and allowed to ...
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As numerical and structural defects in chromosomes are an inevitable consequence of IVF, Pre-implantation genetic diagnosis and screening (PGD/PGS) methods are used for detecting abnormalities in embryos before implantation to the uterus to increase the successful rate of IVF. Pre-implantation genetic diagnosis and screening approaches can be achieved by different techniques such as NGS, CGH an...
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a:4:{s:10:"Background";s:431:"Parental anxiety while waiting for the results of amniocentesis has been investigated by many authors. It seems that the implementation of faster techniques such as fluorescence in-situ hybridization (FISH) will have some benefits in reducing this anxiety. Besides the patients' attitudes to choosing this method, gynecologists who are the persons responsible for tre...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2012
ISSN: 0266-8254
DOI: 10.1111/j.1472-765x.2011.03197.x