Dilemma on plasmid DNA purification: binding capacity vs selectivity

Plasmid DNA chromatography is a powerful field in constant development and evolution. The use of this technique considered mandatory the production an efficient safe formulation to be applied for plasmid-mediated gene therapy. Concerning this, search ideal chromatographic support/ligand combination motivated scientist pursue continuous improvement on plasmid performance, looking progression ligands supports used. present review explores different approaches used over time purify DNA, ambitioning both high recovery purity levels. Overall, it presented critical discussion relying relevance binding capacity versus selectivity supports.