Digital Assays Part II: Digital Protein and Cell Assays
نویسندگان
چکیده
منابع مشابه
Quality control of digital PCR assays and platforms.
Digital polymerase chain reaction (digital PCR, dPCR) is a direct nucleic acid quantification method, thus requiring no standard curves unlike quantitative real-time PCR (qPCR). Nevertheless, evaluation of the linear dynamic range, accuracy, and precision of an assay or platform is recommended, as there are several potential causes of important non-linearity, bias, and imprecision. Ignoring the...
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Synthetic nanoparticles have made significant impact across a broad range of technological applications including optical nanoantennas, ultra-sensitive imaging and sensing, and diagnostics and therapeutics. Natural nanoparticles such as viruses and pollutants are major concerns for human health. High-throughput characterization of nanoparticles in terms of their size and shape is crucial for pr...
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Digital assays are powerful methods that enable detection of rare cells and counting of individual nucleic acid molecules. However, digital assays are still not routinely applied, due to the cost and specific equipment associated with commercially available methods. Here we present a simplified method for readout of digital droplet assays using a conventional real-time PCR instrument to measure...
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The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Thre...
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Parathyroid hormone (PTH) plays a vital role in regulating of blood Calcium. In addition, it regulates the amount of phosphate and vitamin D in the blood. PTH is a polypeptide hormone that found in the blood circulation as peptides with different amino acid counts. The active form of the PTH which has biological activity, contains 84 amino acids, but under the influence of proteolytic enzymes...
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ژورنال
عنوان ژورنال: SLAS TECHNOLOGY: Translating Life Sciences Innovation
سال: 2017
ISSN: 2472-6303,2472-6311
DOI: 10.1177/2472630317705681