Differential Proteolysis of Glycinin and β-Conglycinin Polypeptides during Soybean Germination and Seedling Growth
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منابع مشابه
Differential Proteolysis of Glycinin and beta-Conglycinin Polypeptides during Soybean Germination and Seedling Growth.
The degradation of the major seed storage globulins of the soybean (Glycine max [L.] Merrill) was examined during the first 12 days of germination and seedling growth. The appearance of glycinin and beta-conglycinin degradation products was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cotyledon extracts followed by electroblotting to nitrocellulose and immunostaining...
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The agronomic performance and storage proteins of high seed protein lines of soybeans [Glycine max L. (Merr.)] were investigated to determine if the two major storage proteins, beta-conglycinin and glycinin, contribute to the increased protein content of high seed protein lines. Subunits of these two major storage proteins were estimated by scanning SDS-PAGE gels by scanning densitometry. The r...
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Soybean breeding programs underway today are addressing the goal of improving the protein profile to benefit the human diet as well as that of livestock. Glycinin, a globulin storage protein of the meal and designated as the 11S size fraction by ultracentrifugation, is desirable because of its relative abundance of sulfur-containing amino acids, such as methionine and cysteine. The current stud...
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The coagulation of β-conglycinin (7S), glycinin (11S) and isoflavones induced by calcium chloride was investigated. Approximately 92.6% of the soymilk proteins were coagulated into the soymilk pellet fraction (SPF) after the addition of 5 mM calcium chloride. SDS-PAGE and two-dimensional electrophoresis analysis indicated that most of the 7S (α', α and β), 11S acidic (A1a, A1b, A2, A3 and A4) a...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1986
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.82.1.71