Differential mercury volatilization by tobacco organs expressing a modified bacterial merA gene
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چکیده
منابع مشابه
Mercuric ion reduction and resistance in transgenic Arabidopsis thaliana plants expressing a modified bacterial merA gene.
With global heavy metal contamination increasing, plants that can process heavy metals might provide efficient and ecologically sound approaches to sequestration and removal. Mercuric ion reductase, MerA, converts toxic Hg2+ to the less toxic, relatively inert metallic mercury (Hg0) The bacterial merA sequence is rich in CpG dinucleotides and has a highly skewed codon usage, both of which are p...
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Volatilization of mercury has been observed from various biological media (tissue homogenates, infusion broth, plasma, urine) containing mercuric chloride. That micro-organisms were responsible was indicated by the finding that the rates of volatilization were highly variable, that a latent period often preceded volatilization, that toluene inhibited the process, and that the capacity to volati...
متن کاملmerA gene expression in aquatic environments measured by mRNA production and Hg(II) volatilization.
The relationship of merA gene expression (specifying the enzyme mercuric reductase) to mercury volatilization in aquatic microbial communities was investigated with samples collected at a mercury-contaminated freshwater pond, Reality Lake, in Oak Ridge, Tenn. Levels of merA mRNA transcripts and the rate of inorganic mercury [Hg(II)] volatilization were related to the concentration of mercury in...
متن کاملA conservative region of the mercuric reductase gene (mera) as a molecular marker of bacterial mercury resistance
The most common bacterial mercury resistance mechanism is based on the reduction of Hg(II) to Hg(0), which is dependent of the mercuric reductase enzyme (MerA) activity. The use of a 431 bp fragment of a conservative region of the mercuric reductase (merA) gene was applied as a molecular marker of this mechanism, allowing the identification of mercury resistant bacterial strains.
متن کاملSimplified X-ray film method for detection of bacterial volatilization of mercury chloride by Escherichia coli.
A simplified X-ray film method for detecting bacterial production of Hgo by Escherichia coli was developed. There was excellent agreement of results between this X-ray film method and radioactive mercury volatilization assays. Although not quantitative, the X-ray film method has the advantages of being rapid, simple to perform, and inexpensive.
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ژورنال
عنوان ژورنال: Cell Research
سال: 2001
ISSN: 1001-0602,1748-7838
DOI: 10.1038/sj.cr.7290091