Differential Identification of Mycoplasma pulmonis and M. arthritidis Using PCR-Based RFLP
نویسندگان
چکیده
منابع مشابه
Differential identification of mycoplasma pulmonis and M. arthritidis using PCR-based RFLP.
Mycoplasma pulmonis and Mycoplasma arthritidis were differentially identified using PCR-restriction fragment length polymorphism (RFLP). A genus-specific sequence of mycoplasma was amplified by PCR and the PCR products were digested with the restriction enzyme SmaI. Each PCR product from the four isolates of M. pulmonis was digested with SmaI into two fragments; however, there was no digestion ...
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BACKGROUND & OBJECTIVE We report a new polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) assay using mycobacterial groES as a target to identify Mycobacterium avium and M. intracellulare in clinical samples. METHODS The assay was standardized using M. avium and M. intracellulare standard strains obtained from ATCC and was tested with 45 M. avium-M. intracellula...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 2005
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.54.359