Differential Downstream Functions of Protein Kinase Cη and -θ in EL4 Mouse Thymoma Cells
نویسندگان
چکیده
منابع مشابه
Absence of protein kinase C in nuclei of EL4 mouse thymoma cells.
Since evidence indicates that phorbol ester-induced production of interleukin 2 requires transcription, we investigated the possibility that the phorbol ester receptor acts directly in the nuclei of EL4 thymoma cells. Using a procedure that minimized plasma membrane contamination (as measured by 5'-nucleotidase activity) and maintained the integrity of the double nuclear membrane, we were unabl...
متن کاملAbsence of Protein Kinase C in Nuclei of EL4 Mouse Thymoma Cells1
Since evidence indicates that phorbol ester-induced production of interleukin 2 requires transcription, we investigated the possibility that the phorbol ester receptor acts directly in the nuclei of EL4 thymoma cells. Using a procedure that minimized plasma membrane contamination (as measured by S'-nucleotidase activity) and maintained the integrity of the double nuclear membrane, we were unabl...
متن کاملEffects of protein kinase C activators on phorbol ester-sensitive and -resistant EL4 thymoma cells.
Phorbol ester-sensitive EL4 murine thymoma cells respond to phorbol 12-myristate 13-acetate with activation of ERK mitogen-activated protein kinases, synthesis of interleukin-2, and death, whereas phorbol ester-resistant variants of this cell line do not exhibit these responses. Additional aspects of the resistant phenotype were examined, using a newly-established resistant cell line. Phorbol e...
متن کاملActivation of messenger-independent protein kinases in wild-type and phorbol ester-resistant EL4 thymoma cells.
Phorbol esters, acting via activation of the protein kinase C family of protein serine/threonine kinases, are able to exert profound effects on various cellular functions. In this study, we used the EL4 thymoma cell line to study the potential role of "downstream" protein serine/threonine kinases in cellular responses to phorbol esters. In wild-type EL4 cells, addition of phorbol ester caused a...
متن کاملScreening of interleukin-2 production inhibitor with mouse thymoma EL4 cells.
Sir: Human interleukin-2 (IL-2) regulates many essential immune functions, but its overexpression is causally related to malignancy, autoimmune disease and renal allograft rejection1-3). In order to find immunosuppressants, isotopelabeled enzyme-linked immunosorbent assay (ELISA) and IL-2 reporter gene assay are used to detect IL-2 production, and cyclosporin A (CsA) and FK506 have been found t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.42.27654