Differential Detection of Hemotropic Mycoplasma Species in Cattle by Melting Curve Analysis of PCR Products
نویسندگان
چکیده
منابع مشابه
Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis
Major histocompatibility complex (MHC) represents an important genetic marker for manipulation to improve the health and productivity of cattle. It is closely associated with numerous disease susceptibilities and immune responses. Bovine MHC, also called bovine leukocyte antigen (BoLA), is considered as a suitable marker for genetic diversity studies. In cattle, most of the polymorphisms are lo...
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Cryptosporidium spp. are protozoan parasites that infect various vertebrate hosts. Although 10 species are recognized, at least five types have been found to infect human(1). Thus, the identification of Cryptosporidium spp. and genotypes is important for the assessment of public health issues and for tracking infection or contamination sources(2). Presently, the identification of Cryptosporidiu...
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Both C. pneumoniae and M. pneumoniae are common causes of respiratory tract infection. At present, both are still diagnosed in the laboratory retrospectively by serology. This is despite many publications which indicate that PCR, which is not retrospective, is extremely good at detecting these organisms. We thought that a single PCR test which could detect both organisms simultaneously in a...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2010
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.09-0338