Differential Detection of Five Mouse-Infecting Helicobacter Species by Multiplex PCR

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Differential detection of five mouse-infecting helicobacter species by multiplex PCR.

Several species of helicobacter have been isolated from laboratory mice, including H. bilis, H. hepaticus, H. muridarum, H. rodentium, and H. typhlonius, which appear to be the most common. The most widely used published method for molecular detection of these agents is PCR amplification of a conserved region of 16S rRNA, but differential speciation requires restriction enzyme digestion of the ...

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simultaneous detection of helicobacter genus and helicobacter pylori species using a multiplex pcr method

in order to improve simultaneous detection and identification of helicobacter genus in general and helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex pcr. in this study, two pairs of primers: hcom1 and hcom2 specific for helicobacter genus, hicd1 and hicd2 specific for helicobacter pylori species were used. to determine the sensitivity of...

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ژورنال

عنوان ژورنال: Clinical and Vaccine Immunology

سال: 2005

ISSN: 1556-6811,1556-679X

DOI: 10.1128/cdli.12.4.531-536.2005