Different quiescence states of three culture cell lines detected by acridine orange staining of cellular RNA
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Acridine orange staining for rapid diagnosis of Acanthamoeba keratitis.
Acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. Delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. Therefore, accurate and rapid diagnosis of Acanthamoeba keratitis is essential for successful treatment and good prognosis. We evaluated the usefulness of acr...
متن کاملUse of acridine orange staining for the detection of rotavirus RNA in polyacrylamide gels.
Acridine orange is a metachromatic intercalator used extensively in histochemistry to differentiate double- from single-stranded (ds, ss) nucleic acid by the emission of green and red fluorescence, respectively, under ultraviolet light. In the present study we standardised a protocol in order to use acridine orange to detect rotavirus ds RNA in polyacrylamide gels and compared it to silver and ...
متن کاملHistochemical observations on Mycoplasma after staining with acridine orange.
Mycoplasma colonies and Mycoplasma cells in preparations from infected milk and lymph nodes were observed for their fluorescent qualities after treatment with acridine orange. Mycoplasma colonies fluoresced brilliant red or red-orange. When treated after exposure to ribonuclease, the colonies fluoresced lime-green. There was no fluorescence when both ribonucleic acid and deoxyribonucleic acid w...
متن کاملDynamics of Acridine Orange-cell Interaction
The in vitro localization of acridine orange (AO) in living cells was monitored by means of fluorescence microscopy, quantitative cell viability studies, and photofluorimetric measurements following dye-cell interaction. The parameters, pH, time, dye concentration, and the metabolic state of the cell were found to exert a profound influence on the time course and distribution of staining. The p...
متن کاملStaining with an Acridine Orange Derivative for the Detection of Mycoplasmas in Cell Cultures
• Fischer 0., Dagmar Zendulkova: Staining With an Acridine Orange Derivative for the Detection of Mycoplasmas in Cell Cultures. Acta vet. Brno, 62,1993: 49-53. 24 cell cultures were examined for the presence of mycoplasmas by fluorescence methods using 3-amino-6-methoxy-9-(2-hydroxyethylamino) acridine (AMHA) or bisbenzimide 33258 (Hoechst), and by culture in liquid media containing glucose or ...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1988
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990090409