Different pollination approaches to compare the seed set of diploid and tetraploid red clover <i>Trifolium pratense</i> L.

In red clover seed production, low yield is limiting the commercial exploitation of tetraploid clover. To explore if pollination factor for in clover, we investigated pollinator behaviour and plant reproductive success diploid (2x) cultivar ‘Rajah' (4x) ‘Amos' using honey bee hand approaches. We measured set at flower head level with increasing visitation rate bees (one bee, two open pollination) pollen diversity donors) pollination. found no difference (visitation rate) between Surprisingly, was significantly lower than that one ‘Rajah'. suggest prefer to visit already tripped florets or fewer due scent marks left by previous bees. pollination, number per (on average 11) half 20). Seed 50% treatment) donors treatments). There were differences stigma receptivity whereas viability after peak reached (six days onset flowering). fertility problems such as may be reason This conclusion should verified investigating other traits during ovary, embryo development a broader range cultivars.