Difference between various copper-containing enzymes (Polyporus laccase, mushroom tyrosinase and cucumber ascorbate oxidase) and bilirubin oxidase.
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چکیده
منابع مشابه
Direct electron transfer between copper-containing proteins and electrodes.
The electrochemistry of some copper-containing proteins and enzymes, viz. azurin, galactose oxidase, tyrosinase (catechol oxidase), and the "blue" multicopper oxidases (ascorbate oxidase, bilirubin oxidase, ceruloplasmin, laccase) is reviewed and discussed in conjunction with their basic biochemical and structural characteristics. It is shown that long-range electron transfer between these enzy...
متن کاملBiochemical and structural characterisation of the copper containing oxidoreductases catechol oxidase, tyrosinase, and laccase from ascomycete fungi
Catechol oxidase (EC 1.10.3.1), tyrosinase (EC 1.14.18.1), and laccase (EC 1.10.3.2) are copper-containing metalloenzymes. They oxidise substituted phenols and use molecular oxygen as a terminal electron acceptor. Catechol oxidases and tyrosinases catalyse the oxidation of p-substituted o-diphenols to the corresponding o-quinones. Tyrosinases also catalyse the introduction of a hydroxyl group i...
متن کاملAzide binding to the trinuclear copper center in laccase and ascorbate oxidase.
Azide binding to the blue copper oxidases laccase and ascorbate oxidase (AO) was investigated by electron paramagnetic resonance (EPR) and pulsed electron-nuclear double resonance (ENDOR) spectroscopies. As the laccase : azide molar ratio decreases from 1:1 to 1:7, the intensity of the type 2 (T2) Cu(II) EPR signal decreases and a signal at g approximately 1.9 appears. Temperature and microwave...
متن کاملGalactose oxidase of Polyporus circinatus: a copper enzyme.
In 1959, Cooper et al. (1) described the isolation of an enzyme from Polyporus circinatus Fr. which catalyzed the oxidation of galactose by molecular oxygen. MTe have previously described a procedure for the purification of such an enzyme from culture filtrat,es and have shown the reaction product to be D-g&CtOhexodialdose, oxidation occurring at the C-6 rather than the C-l position (2). The pr...
متن کاملAscorbate oxidase.
A new procedure has been described for the isolation and purification of ascorbate oxidase from green zucchini squash (Cucurbita pepo meduttosa). The new purification method achieves a more than ZOO-fold purification with about a 14% recovery of the total activity present in the crude juice. The purified enzyme preparations have been found to be homogeneous by the criteria of ultracentrifugal a...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1983
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.47.1627