Dichloran-glycerol medium for enumeration of xerophilic fungi from low-moisture foods

Species of Drechslera producing metabolites

Fungi which grow in stored commodities are xerophilic, that is, capable of growing at reduced water activity (aw). Some xerophilic fungi either will not grow or grow only very slowly at the high aw values of media traditionally used to enumerate yeasts and moulds in foods and feedstuffs. Populations of xerophiles may therefore be underestimated or go undetected if the media and methods normally...

Evaluation of Chemicals for Restricting Colony Spreading by a Xerophilic Mold, Eurotium amstelodami, on Dichloran-18% Glycerol Agar.

Twenty chemicals were screened for their effectiveness in restricting colony spreading by four strains of a xerophilic mold, Eurotium amstelodami, on dichloran-18% glycerol agar. Triton X-100, Triton X-301, Tergitol NP-7, and Tergitol 15-S-3 (each at 200 mug/ml) and 1,000 mug of sodium deoxycholate, 1 mug of iprodione, 0.1 mug of propiconazole, and 0.01 mug of Maxim per ml were judged to be mos...

Annex to Control of Salmonella in Low-moisture Foods

New medium for rapid screening and enumeration of Clostridium perfringens in foods.

A rapid and sensitive procedure for estimating low numbers of Clostridium perfringens has been investigated and compared to methods used currently in the food industry. The new liquid medium, RPM (rapid perfringens medium), was compared with sulfite-polymyxin-sulfadiazine agar and tryptose-sulfite-cycloserine agar in recovery studies with naturally contaminated and with inoculated foods. The me...

Rose bengal-malt extract-agar, a simple medium for the simultaneous isolation and enumeration of fungi and actinomycetes from soil.

merous than fungi, these organisms used to be suppressed by acidifying the agar medium to pH 4 to 5, but this has been made unnecessary by the addition of rose bengal to suitable media (N. R. Smith and V. T. Dawson, Soil Sci. 58:467, 1944). Counts of fungi were even higher in media containing rose bengal than in acidified media (J. P. Martin, Soil Sci. 69:215, 1960). Actinomycetes grow more slo...