Dialogue between centrosomal entrance and exit scaffold pathways regulates mitotic commitment

Dialogue between centrosomal entrance and exit scaffold pathways regulates mitotic commitment

The fission yeast scaffold molecule Sid4 anchors the septum initiation network to the spindle pole body (SPB, centrosome equivalent) to control mitotic exit events. A second SPB-associated scaffold, Cut12, promotes SPB-associated Cdk1-cyclin B to drive mitotic commitment. Signals emanating from each scaffold have been assumed to operate independently to promote two distinct outcomes. We now fin...

Relation between entrance and exit pupils of telescopic systems.

Regulation of CDC14: pathways and checkpoints of mitotic exit.

Progression of the mitotic cell cycle is driven by fluctuations of the cyclin-dependent kinase (Cdk) activities. Entry into mitosis is promoted by the elevated activity of Cdk1 associated with B-type cyclins. Conversely, exit from mitosis requires the inactivation of Cdk1 and the dephosphorylation of at least a subset of Cdk1 substrates. The Cdc14 family of phosphatases antagonizes the action o...

Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae.

In budding yeast Saccharomyces cerevisiae, Cdc5 kinase is a component of mitotic exit network (MEN), which inactivates cyclin-dependent kinase (CDK) after chromosome segregation. cdc5-1 mutants arrest at telophase at the nonpermissive temperature due to the failure of CDK inactivation. To identify more negative regulators of MEN, we carried out a genetic screen for genes that are toxic to cdc5-...

Cdc7p-Dbf4p Regulates Mitotic Exit by Inhibiting Polo Kinase

Cdc7p-Dbf4p is a conserved protein kinase required for the initiation of DNA replication. The Dbf4p regulatory subunit binds Cdc7p and is essential for Cdc7p kinase activation, however, the N-terminal third of Dbf4p is dispensable for its essential replication activities. Here, we define a short N-terminal Dbf4p region that targets Cdc7p-Dbf4p kinase to Cdc5p, the single Polo kinase in budding ...