Diagnostic value of microRNA?125b in peripheral blood mononuclear cells for pulmonary tuberculosis

Pulmonary tuberculosis (TB) is a chronic respiratory infectious disease. Certain microRNAs (miRNAs or miRs) are reported to be involved in regulating TB progression. The present study aimed evaluate the diagnostic potential of miR?125b pulmonary TB. expression levels and Raf1 proto?oncogene serine/threonine protein kinase (RAF1) were analyzed via reverse transcription?quantitative (RT?q)PCR patients with correlation between clinical indicators was investigated, receiver operating characteristic (ROC) curve used value miR?125b. relationship RAF1 examined using dual luciferase reporter gene assay. IL?6, TNF??, NF??B IFN?? detected ELISA kits, then NF??B, peripheral blood mononuclear cells (PBMCs) analyzed. Moreover, mRNA RT?qPCR western blotting. results demonstrated that decreased TB, while increased. Furthermore, associated sputum acid?fast bacillus smear. area under ROC 0.9413, sensitivity specificity for 90 92.5%, respectively. negatively correlated expression, inhibited by PBMCs. targeted regulate its levels. reversed role attenuating Thus, it suggested served important roles occurrence development decreasing inhibiting RAF1.