Development of the first PVM TaqMan® primer set and a one-step real-time multiplex DiRT-PCR for the detection of PLRV, PVY, PVM, PVS, PVA and PVX in potato tuber sap

Abstract Testing for potato viruses is globally very important to prevent a critical shortage of supply. In most countries, testing obligated by law. Germany, seed potatoes are monitored six viruses: PLRV, PVY, PVM, PVA, PVX and PVS. They can cause up 90% loss tubers in the field. Common methods currently used ELISA conventional real-time PCR, but both time-consuming, former needs high capacity green houses human resources, latter elaborate RNA extraction steps. Recently, we proposed new method called DiRT-PCR which enables us test PVY PVS along with an internal control three duplex PCR reactions directly on diluted tuber sap. this study, describe first TaqMan® assay PVM published so far embed it into multiplex system detect remaining viruses. We now able sensitively presence two using without purification.