Development of SSR marker by RNA-seq and its application in genotyping pearl sac in pearl oyster Pinctada fucata martensii
نویسندگان
چکیده
منابع مشابه
The pearl oyster Pinctada fucata martensii genome and multi-omic analyses provide insights into biomineralization
Nacre, the iridescent material found in pearls and shells of molluscs, is formed through an extraordinary process of matrix-assisted biomineralization. Despite recent advances, many aspects of the biomineralization process and its evolutionary origin remain unknown. The pearl oyster Pinctada fucata martensii is a well-known master of biomineralization, but the molecular mechanisms that underlie...
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We examined the effect of tributyltin (TBT) on reproduction of the pearl oyster (Pinctada fucata martensii). In a maternal exposure test, five female pearl oysters were exposed to TBT at measured concentrations of 0 (control), 0.092, or 0.191 microg/L at 25 degreesC for one week, and the embryo developmental success (the ratio of normal D-larvae to all larvae) was measured. The embryo developme...
متن کاملGenetic linkage map of the pearl oyster, Pinctada martensii (Dunker)
Genetic linkage maps were constructed with ampli¢ed fragment length polymorphism (AFLP) and microsatellite markers for the pearl oyster, Pinctada martensii (Dunker), the main bivalve used for marine pearl production in Asia. Twenty-four AFLP and 84 microsatellite primer pairs were used for linkage analysis in a full-sib family with two parents and 78 o¡spring. Of the 2357 AFLP fragments generat...
متن کاملMicroRNA, Pm-miR-2305, Participates in Nacre Formation by Targeting Pearlin in Pearl Oyster Pinctada martensii
MicroRNAs (miRNAs) are noncoding RNA molecules that function as negative regulators of target genes. In our previous research, 258 pm-miRNAs were identified in Pinctada martensii by Solexa deep sequencing. Pm-miR-2305 was one of the identified pm-miRNAs with a potential function in biomineralization. In the present study, the precursor of pm-miR-2305 was predicted with 96 bp, containing a chara...
متن کاملIdentification of Genes Directly Involved in Shell Formation and Their Functions in Pearl Oyster, Pinctada fucata
Mollusk shell formation is a fascinating aspect of biomineralization research. Shell matrix proteins play crucial roles in the control of calcium carbonate crystallization during shell formation in the pearl oyster, Pinctada fucata. Characterization of biomineralization-related genes during larval development could enhance our understanding of shell formation. Genes involved in shell biomineral...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2017
ISSN: 0717-3458
DOI: 10.1016/j.ejbt.2016.11.004