Development of Split-Protein Systems: From Binary to Ternary System

Tens of thousands protein-protein interactions (PPIs) have been found in human cells and many these macromolecular partnerships could determine the cell growth death. Thus there is a need to develop methods catalogue macromolecules by detecting their interactions, modifications, cellular locations. It will be helpful for scientists compare difference between diseased state its normal find potential therapy treatment intervene this status. One technology called split-protein reassembly or protein fragment complementation has developed last two decades. This makes use appropriate fragmentation some reporters refolding reports detected function confirm interaction interest. system set up cell-free systems, E. coli, yeast, mammalian cells, plants live animals. Herein, I present development fluorescence- bioluminescence-based biosensors both binary ternary systems. In addition, people combining it with chemical inducer dimerization strategy (CID). applied identifying enzyme inhibitors regulating activity kinases phosphatases. With effort from laboratories world, variety systems studying PPI vitro vivo, monitoring biological process, controlling