Development of single nucleotide polymorphism‐based functional molecular markers from the <i>Lr22a</i> gene sequence in wheat ( <scp> <i>Triticum aestivum</i> </scp> )

The adult-plant leaf rust resistant gene Lr22a confers broadly effective resistance against the fungal pathogen Puccinia triticina Eriks. (Pt) in wheat that has not been extensively utilized cultivars. objective of this study was to develop robust functional molecular markers using sequence facilitate integration breeding programmes. coding used identify isolated SNPs and four kompetitive allele specific polymerase chain reaction (PCR) (KASP) were developed. For marker testing, a F2:3 population developed by crossing near-isogenic line RL4495 (Lr22a carrier) with Thatcher phenotyped Pt race TDBJ genotyped 90K iSelect SNP array, KASP two SSR markers. A linkage map chromosome arm 2DS included constructed. co-segregated validated for cross-applicability on panel lines. Kwh636, Kwh637 Kwh638 reliably detected presence or absence Lr22a. will selection