Development of real-time reverse transcriptase qPCR assays for the detection of Punta Toro virus and Pichinde virus
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چکیده
منابع مشابه
Development of real-time reverse transcriptase PCR assays for the detection of Punta Toro
29 30 Background: Research with high biocontainment pathogens such as Rift Valley fever virus 31 (RVFV) and Lassa virus (LASV) is expensive, potentially hazardous, and limited to select 32 institutions. Surrogate pathogens such as Punta Toro virus (PTV) for RVFV infection and 33 Pichinde virus (PICV) for LASV infection allow research to be performed under more 34 permissive BSL-2 conditions. Al...
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We have investigated the oligomerization and intracellular transport of the membrane glycoproteins of Punta Toro virus, a member of the Phlebovirus genus of the family Bunyaviridae, which is assembled by budding in the Golgi complex. By using one- or two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, chemical cross-linking, and sucrose gradient centrifugation, we found t...
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Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in people in many developing countries and is also endemic in many industrialized countries. Mammalian HEV (mHEV) isolates can be divided into at least four recognized major genotypes. Several nucleic acid amplification techniques have been developed for mHEV detection, with great differences in sensitivity. The aim of this study...
متن کاملComparison of real - time reverse transcriptase ( RT ) - PCR assays for 1 detection of swine hepatitis E virus in fecal samples
5 a Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 6 Medicine, Iowa State University, Ames, Iowa, USA 7 b Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária 8 Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Brazil 9 Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine ...
متن کاملDevelopment of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2016
ISSN: 1743-422X
DOI: 10.1186/s12985-016-0509-3