Development of enzyme immunoassay for erythrocyte aldose reductase.
نویسندگان
چکیده
منابع مشابه
Enzyme immunoassay for erythrocyte aldose reductase.
This two-site immunoassay measures erythrocyte aldose reductase by using monoclonal and polyclonal antibodies to recombinant human enzyme. Total incubation time is 2.5 h, and the limit of detection is < 0.05 microgram/L. Analytical recovery tested with blood samples from healthy and diabetic individuals was 101-106%. Average CVs within and between assays were 3.7% and 4.8%, respectively. The en...
متن کاملAldose Reductase Activity from Erythrocyte of Diabetes Mellitus Patients
Diabetes often referred to by doctors as diabetes mellitus, describe a group of metabolic diseases in which the person has high blood glucose (blood sugar), either because insulin production is inadequate, or because the body’s cells do not respond properly to insulin or both. The acceleration of the polyol pathway elicits various metabolic imbalances in those tissues that undergo insulin depen...
متن کاملActivated and unactivated forms of human erythrocyte aldose reductase.
Aldose reductase (alditol:NADP+ 1-oxidoreductase, EC 1.1.1.21) has been partially purified from human erythrocytes by DEAE-cellulose (DE-52) column chromatography. This enzyme is activated severalfold upon incubation with 10 microM each glucose 6-phosphate, NADPH, and glucose. The activation of the enzyme was confirmed by following the oxidation of NADPH as well as the formation of sorbitol wit...
متن کاملCorrelation between erythrocyte aldose reductase level and human diabetic retinopathy.
AIM To examine the relation between aldose reductase (AR) and the development and progression of diabetic retinopathy by comparing the erythrocyte AR levels with the prevalence of diabetic retinopathy in NIDDM patients. METHODS A clinic based cross sectional study was used. 611 NIDDM patients and 73 controls were enrolled. Erythrocyte AR levels were determined by ELISA. These AR levels were t...
متن کاملErythrocyte aldose reductase activity and sorbitol levels in diabetic retinopathy
PURPOSE Activation of polyol pathway due to increased aldose reductase (ALR2) activity has been implicated in the development of diabetic complications including diabetic retinopathy (DR), a leading cause of blindness. However, the relationship between hyperglycemia-induced activation of polyol pathway in retina and DR is still uncertain. We investigated the relationship between ALR2 levels and...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1994
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)50942-8