Development of Enantioselective Polyclonal Antibodies to Detect Styrene Oxide Protein Adducts
نویسندگان
چکیده
منابع مشابه
Development of polyclonal antibodies for the detection of styrene oxide modified proteins.
Styrene is widely used as one of the most important industrial materials for the production of synthetic rubbers, plastic, insulation, fiberglass, and automobile parts. Inhaled styrene has been reported to produce respiratory toxicity in humans and animals. Styrene oxide, a reactive metabolite of styrene formed via cytochrome P450 enzymes, has been reported to form covalent bonds with proteins,...
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Hemoglobin and albumin adducts of the carcinogen styrene-7,8-oxide (SO) were measured in 48 workers exposed to both styrene and SO in a boat manufacturing plant. Personal exposures to both substances were measured repeatedly over the course of 1 year (styrene:0.9-235 mg/m3 with a mean of 64.3 mg/m3 for 48 subjects; S0: 13.4-525 mu g/m3 with a mean of 159 mu g/m3 for 20 subjects). Cysteine and c...
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Immunization of mice with acetaldehyde conjugated to human plasma proteins resulted in the production of polyclonal antibodies that reacted with erythrocyte protein-acetaldehyde conjugates, but not with control erythrocyte proteins. Such antibodies recognized erythrocyte protein-acetaldehyde conjugates prepared with 20-100 microM acetaldehyde, concentrations that exist in the blood of alcoholic...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2009
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac8023262