Development of diagnostic SNP markers for identification of rye 1RS translocations in wheat

Abstract Wheat ( Triticum aestivum L.) wild relatives are rich genetic resources for improvement of wheat. The short arm rye Secale cereale chromosome 1 (1RS) contains many beneficial genes resistance to drought, insects, and diseases, therefore the 1RS wheat translocations have been widely used in breeding programs worldwide. To facilitate marker‐assisted identification breeding, we designed a set Kompetitive allele‐specific polymerase chain reaction (KASP) markers detect with long from 1A (1RS.1AL) 1B (1RS.1BL) based on sequence flanking single nucleotide polymorphisms (SNPs) generated by genotyping‐by‐sequencing (GBS) between panels. We identified three SNP diagnosis differentiation two Among KASP markers, 1AS‐05056 is specific, clearly separated genotypes (1RS.1AL 1RS.1BL) those without translocations; whereas 1AS‐29875 1RS.1AL translocation 1BS‐16654 1RS.1BL 1RS.1AL. new together accurately presence either or panel 95 representative U.S. winter accessions, which were further validated additional hard Therefore, they can be routine screening lines programs.