Development of Bottle Gourd Lines Resistant to Zucchini Yellow Mosaic Virus Using Ethyl Methanesulfonate Mutagenesis
نویسندگان
چکیده
Zucchini yellow mosaic virus (ZYMV) causes serious damage to cucurbit crops worldwide and can be spread by aphids, mechanical injury, in seeds. With the popularization of grafting, use susceptible rootstock has increased risk ZYMV infection crops. In China, bottle gourd ( Lagenaria siceraria ) is a widely used grafted watermelon production. However, few resistant gourds are available commercially. This study developed lines using ethyl methanesulfonate (EMS) mutagenesis. A new mutated population (M 1 was generated treating seeds with EMS. Diverse phenotypes were observed seedlings, flowers, fruit M 2 plants, some which potential commercial interest, such as dwarfing different shapes. Based on phenotypes, 106 3 selected screened for resistance inoculation agroinfiltration. Nine during three tests. One inbred 4 line (177-8) showed stable no when tested double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) polymerase chain reaction. These promising materials developing exploring genes ZYMV-resistant resources. EMS induction could practical strategy creating
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ژورنال
عنوان ژورنال: Hortscience
سال: 2021
ISSN: ['0018-5345', '2327-9834']
DOI: https://doi.org/10.21273/hortsci15898-21