Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

The apicomplexan parasite Theileria haneyi is one of two known causative agents equine theileriosis. It causes milder clinical disease than its more virulent counterpart, equi, in experimentally infected horses, and can superinfect T. equi-positive horses. current equi merozoite antigen 1 (EMA1)-based competitive enzyme-linked immunosorbent assay (ELISA)used the U.S. to detect theileriosis detects but not haneyi, complexity molecular assays precludes widespread use for epidemiologic studies. In order facilitate urgently needed studies on prevalence goal this study was develop a sensitive specific serologic diagnosis based 11 (ThEMA11). To achieve objective, ThEMA11 recombinantly expressed eukaryotic cells antigenicity assessed using sera from haneyi-experimentally Confirmation reactivity enabled design optimization an indirect ELISA. Specificity ELISA cohort horses confirmed PCR-positive either or haneyi. Data field samples further demonstrate that capable identifying antibodies multiple continents around world.