Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in Subsurface Environments
نویسندگان
چکیده
منابع مشابه
Fluorescent Staining Method for Bacterial Endospores
We have developed a quick, simple technique for the fluorescent staining of bacterial endospores. This technique was developed using Bacillus subtilis endospores. Our technique requires minimal sample heating so it can safely be carried out entirely in a glove-box. The endospores stain diagnostically as an oval with a standard fluorescent nucleotide stain, acridine orange.
متن کاملComparison of methods for monitoring bacterial transport in the subsurface.
The purpose of this study was to compare in a laboratory experiment, a suite of methods developed to track viable bacteria during field transport experiments. The criteria for development and selection of these methods included: (1) the ability to track bacteria within the environment from which they were isolated; (2) the lack of any effect upon the viability or the transport characteristics o...
متن کاملApplication of a vital fluorescent staining method for simultaneous, near-real-time concentration monitoring of two bacterial strains in an Atlantic coastal plain aquifer in Oyster, Virginia.
Two differentially labeled bacterial strains were monitored in near-real time during two field-scale bacterial transport experiments in a shallow aquifer in July 2000 and July 2001. Comamonas sp. strain DA001 and Acidovorax sp. strain OY-107 were grown and labeled with the vital fluorescent stain TAMRA/SE (5 [and -6]-carboxytetramethylrhodamine, succinimidyl ester) or CFDA/SE (5 [and -6]-carbox...
متن کاملdevelopment and implementation of an optimized control strategy for induction machine in an electric vehicle
in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...
15 صفحه اولImmunofluorescent Staining: the Fluorescent Antibody Method.
I. Principles of Tmmunofluorescent Staining.................................................. A. Fluorescent Antibody Labeling........................................................ B. Immunofluorescent Staining Procedures............................................... C. Controls.............................................................................. D. Nonspecific Staining................
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2000
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.66.10.4486-4496.2000