DEVELOPMENT OF A SERUM-FREE MEDIUM AND PRIMARY CULTURE OF HUMAN RENAL CELL CARCINOMAS BY SERUM-FREE CULTURE
نویسندگان
چکیده
منابع مشابه
[Development of a serum-free medium and primary culture of human renal cell carcinomas by serum-free culture].
Ovomacroglobulin and LDL fraction of egg yolk were effective to maintain the growth of established human cancer cell lines (Kato III, QG-90, Raji). A serum-free medium (BEM-841) containing 10 mM HEPES, 50 micrograms/ml of ovomacroglobulin, 0.1% (V/V) of LDL fraction of egg yolk, 10(-8) M catalase, 5 micrograms/ml of insulin, 5 micrograms/ml of transferrin, 5 ng/ml of selenium, 1 mg/ml of BSA, a...
متن کاملCulture of Lymphocytes and Hemopoietic Cells in Serum-Free Medium
Freshly explanted lymphocytes and hemopoietic cells can grow and differentiate to terminally functioning cells in culture. Such cultures provide an opportunity to study, in cells not previously subjected to in vitro selection, such phenomena as induction of growth in physiologically resting cells, immune recognition, and the function of specific polypeptide factors and cell surface receptors. T...
متن کاملModulating the development of renal tubules growing in serum-free culture medium at an artificial interstitium.
Little information on the structural growth of renal tubules is available. A major problem is the technical limitation of culturing intact differentiated tubules over prolonged periods of time. Consequently, we developed an advanced culture method to follow tubule development. Isolated tissue containing renal progenitor cells was placed in a perfusion culture container at the interphase of an a...
متن کاملContinuous culture of rat C6 glioma in serum-free medium
In this communication we describe serum-free culture conditions for the serial propagation of the C6 glioma cell line. The growth rate, saturation density, and morphology of these cells are equivalent to those of their serum-grown counterparts when cultured in a 3:1 mixture of Dulbecco's modified Eagle's medium and Ham's medium F-12 supplemented with trace elements, insulin, transferrin, fibrob...
متن کاملInvestigation and Application Progress of Vero Cell Serum-free Culture
Serum-free culture is now becoming the general trend of biological production. Its application in Vero cell culture is significant. In the present paper, we reviewed factors affecting Vero cell serum-free culture and several culture techniques. In light of these reviews, we outlined its extensive application prospect.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Japanese Journal of Urology
سال: 1989
ISSN: 0021-5287,1884-7110
DOI: 10.5980/jpnjurol1989.80.1741