Development of a replication-competent lentivirus assay for dendritic cell-targeting lentiviral vectors
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چکیده
منابع مشابه
Development of a replication-competent lentivirus assay for dendritic cell-targeting lentiviral vectors
It is a current regulatory requirement to demonstrate absence of detectable replication-competent lentivirus (RCL) in lentiviral vector products prior to use in clinical trials. Immune Design previously described an HIV-1-based integration-deficient lentiviral vector for use in cancer immunotherapy (VP02). VP02 is enveloped with E1001, a modified Sindbis virus glycoprotein which targets dendrit...
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Retroviral vectors based on murine leukemia viruses (MuLV) have been used in clinical investigations for over a decade. Alternative retroviruses, most notably vectors based on HIV-1 and other lentiviruses, are now entering into clinical trials. Although vectors are designed to be replication defective, recombination events during vector production could lead to the generation of replication com...
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[This corrects the article DOI: 10.1016/j.omtm.2017.09.001.].
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Lentiviral vectors (LVs) are efficient gene delivery vehicles suitable for delivering long-term transgene expression in various cell types. Engineering LVs to have the capacity to transduce specific cell types is of great interest to advance the translation of LVs toward the clinic. Here we provide an overview of innovative approaches to target LVs to cells of the immune system. In this overvie...
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ژورنال
عنوان ژورنال: Molecular Therapy - Methods & Clinical Development
سال: 2015
ISSN: 2329-0501
DOI: 10.1038/mtm.2015.17