Development of a novel single tube nested PCR for enhanced detection of cytomegalovirus DNA from dried blood spots
نویسندگان
چکیده
منابع مشابه
Development of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA
Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...
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We have developed an efficient and simple method for extracting and purifying genomic DNA from dried blood stored on filter paper. The quality of the genomic DNA extracted is tested by PCR amplification of a 255-bp fragment of the PAX8 gene sequence and the PCR products are determined for further genetic studies by single strand conformation polymorphism (SSCP) analysis. Larger DNA sequences of...
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T h e collection of dried blood spots on filter paper blotters is a major innovation and has facilitated the development and dissemination of newborn screening programs principally because of the ease of sample collection, handling, and shipment to centrally located laboratories and the stability of the analytes in these specimens. (1,2) These samples, which are also known as "Guthrie spots," a...
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process, and they work together to effect the amniochorionic system that is the main source for production not only of IL-8 (13 ) but also of various prostanoids (14, 15). Therefore, the highly variable concentrations of IL-8 in the CVS may be attributable to the extent of and/or the stages in which these factors contribute to cervical-decidualamniochorionic activation. However, the precise mec...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2014
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2013.10.029