Development of a fluorescent substrate to measure hyaluronidase activity
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چکیده
منابع مشابه
Development of A Questionnaire to Measure Attitude toward Oocyte Donation
Background To our knowledge, there is no valid and comprehensive questionnaire that considers attitude toward oocyte donation (OD). Therefore this study has aimed to design and develop a tool entitled attitude toward donation-oocyte (ATOD-O) to measure attitude toward OD. MaterialsAndMethods This methodological, qualitative research was undertaken on 15 infertile cases. In addition, we performe...
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متن کاملColorimetric determination of hyaluronidase activity.
Turbidimetric methods for assay of hyaluronidase activity are based upon the measurement of the optical density of suspensions of substrateprotein combination in an acid environment (1). The reliability of this measurement depends upon careful control of the conditions under which turbidity develops (2), and even then the relation between substrate concentration and optical density is linear ov...
متن کاملA fluorescent assay to quantitatively measure in vitro acyl CoA:diacylglycerol acyltransferase activity.
Triacylglycerols (TG) are the major storage form of energy in eukaryotic organisms and are synthesized primarily by acyl CoA:1,2-diacylglycerol acyltransferase (DGAT) enzymes. In vitro DGAT activity has previously been quantified by measuring the incorporation of either radiolabeled fatty acyl CoA or diacylglycerol (DG) into TG. We developed a modified acyltransferase assay using a fluorescent ...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2008
ISSN: 0003-2697
DOI: 10.1016/j.ab.2008.04.040