Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy
نویسندگان
چکیده
منابع مشابه
Vector-swapping strategy with an exonuclease in cloning and high expression of small PCR products.
The need for designing new peptides of biological, pharmacological or clinical importance is increasing. To fulfill this need, a detailed knowledge of the structure-function relationship of known bioactive proteins is a prerequisite, and to obtain such information, we face challenges of inserting and deleting specific sequences into or from known proteins and expressing these modified proteins ...
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in this thesis our aim is to construct vector field in r3 for which the corresponding one-dimensional maps have certain discontinuities. two kinds of vector fields are considered, the first the lorenz vector field, and the second originally introced here. the latter have chaotic behavior and motivate a class of one-parameter families of maps which have positive lyapunov exponents for an open in...
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ژورنال
عنوان ژورنال: AMB Express
سال: 2017
ISSN: 2191-0855
DOI: 10.1186/s13568-017-0386-1