Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay
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چکیده
منابع مشابه
Development of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
متن کاملdevelopment of sybr green i based real-time rt-pcr assay for specific detection of watermelon silver mottle virus
background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...
متن کاملDevelopment of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tube...
متن کاملA Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
A one-step SYBR Green I real-time RT-PCR assay was developed for the detection and quantification of a broad range of murine noroviruses (MNVs). The primer design was based on the multiple sequence alignments of 101 sequences of the open reading frame (ORF)1-ORF2 junction of MNV. The broad reactivity and quantitative capacity of the assay were validated using 7 MNV plasmids. The assay was compl...
متن کاملDevelopment of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus.
BACKGROUND Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. OBJECTIVES Development of a highly sensitive and reliable detection method for WSMoV. MATERIALS AND METHODS Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
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ژورنال
عنوان ژورنال: BioMed Research International
سال: 2018
ISSN: 2314-6133,2314-6141
DOI: 10.1155/2018/5035139