Development of a certified reference material for the analysis of vitamins in multivitamin tablets

Abstract Multivitamin tablet certified reference material (CRM, 108-10-019) was developed for the analysis of seven water-soluble vitamins, including thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxine, biotin, and folic acid. The CRM prepared in powder form by grinding multivitamin tablets then mixing, sieving, bottling powder. For certification each vitamin, isotope dilution mass spectrometry based on liquid chromatography applied. methods analyte were validated confirming repeatability reproducibility comparing with other CRMs. property values uncertainties vitamins determined 10 units from sample stored at − 20 °C. homogeneity component also examined range 0.48–2.2%. All stable 3 or 6 years after initial under storage conditions fat-soluble retinol, α-tocopherol, cholecalciferol, phylloquinone, two expert laboratories participated analyses official methods, mean reported results assigned as values. (108-10-019) will be useful validating analytical ensuring quality vitamin similar products.