Development of 30 SNP markers for the Daphnia magna based on restriction-site associated DNA sequencing (RAD-seq)

Daphnia magna belongs to the Cladocera and plays an important role in water ecosystem. With intensification of pollution, wild population D. has declined rapidly recent years, insufficient molecular markers have limited effective research conservation this species. In research, 30 novel single nucleotide polymorphism (SNP) were developed a cultivar 12 using restriction site-associated DNA sequencing (RAD-seq). The minor allele frequency, observed heterozygosity, expected heterozygosity ranged from 0.115 0.483, 0.073 0.800, 0.077 0.520, respectively. PIC 0.071 0.403. Six loci showed significant deviations Hardy-Weinberg equilibrium after Bonferroni correction (p < 0.05).These newly polymorphic SNP for are great significance terms genetic breeding magna, identification artificially domesticated species genetics research.