Development and optimization of orthotopic liver metastasis xenograft mouse models in uveal melanoma
نویسندگان
چکیده
منابع مشابه
Uveal Melanoma Metastasis Models.
Metastatic disease is the leading cause of death among patients with uveal melanoma. Treatment options for patients with clinically disseminated disease are usually unsuccessful. In vitro and in vivo models are important tools to investigate the pathogenesis of metastatic uveal melanomas and develop treatments for the metastases. In vitro experimental approaches focusing on cell invasion/migrat...
متن کاملPatient-Derived Orthotopic Xenograft (PDOX) Models of Melanoma †
Metastatic melanoma is a recalcitrant tumor. Although "targeted" and immune therapies have been highly touted, only relatively few patients have had durable responses. To overcome this problem, our laboratory has established the melanoma patient-derived orthotopic xenograft (PDOX) model with the use of surgical orthotopic implantation (SOI). Promising results have been obtained with regard to i...
متن کاملMolecular pathways mediating liver metastasis in patients with uveal melanoma.
Uveal melanoma arises from melanocytes located in the uveal tract of the eye and is the most common primary intraocular tumor in adults. Metastatic liver disease is the overwhelming cause of death in uveal melanoma patients, with almost 50% of patients developing liver metastases up to 15 years after diagnosis. Most of these patients do not present with any evidence of overt metastasis at the t...
متن کاملFluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models
We report here the development of fluorescence-guided surgery of liver metastasis. HT29 human colon cancer cells expressing green fluorescent protein (GFP) were initially injected in the spleen of nude mice. Three weeks later, established liver metastases were harvested and implanted on the left lobe of the liver in other nude mice in order to make an orthotopic liver metastasis model. Fourteen...
متن کاملModeling of human uveal melanoma in zebrafish xenograft embryos.
PURPOSE Uveal melanoma (UM) is fatal in up to 50% of patients because of liver metastases that are refractory to therapies currently available. While murine xenograft models for human uveal melanoma are available, they have limited utility for screening large compound libraries in drug discovery studies. Therefore, new robust preclinical models are needed that can efficiently evaluate drug effi...
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ژورنال
عنوان ژورنال: Journal of Translational Medicine
سال: 2020
ISSN: 1479-5876
DOI: 10.1186/s12967-020-02377-x