Development and Evaluation of a Polymerase Chain Reaction Assay Using the 16S rRNA Gene for Detection of Eperythrozoon Suis Infection

Polymerase Chain Reaction of Mgc2 and 16S rRNA Genes for Detection of Mycoplasma gallisepticum

Mycoplasmas are very small bacteria lacking cell walls that belong to various genera within the class Mollicutes, and also the smallest organisms that can live independently. They are able to cause serious and chronic disease because of some unique characteristics. Mycoplasma gallisepticum (MG) is an important avian pathogen causing significant economical losses within the poultry industry. The...

Actinobaculum suis Detection Using Polymerase Chain Reaction

Actinobaculum suis is an important agent related to urinary infection in swine females. Due to its fastidious growth characteristics, the isolation of this anaerobic bacterium is difficult, thus impairing the estimation of its prevalence. The purpose of this study was to develop and test a polymerase chain reaction (PCR) for the detection and identification of A. suis and then compare these res...

Detection of Babesia ovis Using Polymerase Chain Reaction.

Polymerase chain reaction assay targeting nox gene for rapid identification of Brachyspira canis in dogs

Genus Brachyspira,as Gram negative anaerobic bacteria, colonize in dogs intestine. The aim of the current study was to determine the prevalence of Brachyspira spp. for the first time in Iran and rapid identification of Brachyspira spp. in dogs by a new designment of a species-specific primer set for B. canis. One hundred fifty-one fecal samples were obtained ...

Development of Multiplex Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Influenza A, B and Adenoviruses

Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. ...