Development and Characterization of EST-SSR Markers for Artocarpus hypargyreus (Moraceae)
نویسندگان
چکیده
منابع مشابه
Development and characterization of EST-SSR markers for Artocarpus hypargyreus (Moraceae)1
PREMISE OF THE STUDY Polymorphic microsatellite markers were developed for Artocarpus hypargyreus (Moraceae), a threatened species endemic to China, to investigate the genetic diversity and structure of the species. METHODS AND RESULTS Based on the transcriptome data of A. hypargyreus, 63 primer pairs were preliminarily designed and tested, of which 34 were successfully amplified and 10 displ...
متن کاملDevelopment and Characterization of Polymorphic EST-SSR and Genomic SSR Markers for Tibetan Annual Wild Barley
Tibetan annual wild barley is rich in genetic variation. This study was aimed at the exploitation of new SSRs for the genetic diversity and phylogenetic analysis of wild barley by data mining. We developed 49 novel EST-SSRs and confirmed 20 genomic SSRs for 80 Tibetan annual wild barley and 16 cultivated barley accessions. A total of 213 alleles were generated from 69 loci with an average of 3....
متن کاملNew development and validation of 50 SSR markers in breadfruit (Artocarpus altilis, Moraceae) by next-generation sequencing1
PREMISE OF THE STUDY Using next-generation sequencing technology, new microsatellite loci were characterized in Artocarpus altilis (Moraceae) and two congeners to increase the number of available markers for genotyping breadfruit cultivars. METHODS AND RESULTS A total of 47,607 simple sequence repeat loci were obtained by sequencing a library of breadfruit genomic DNA with an Illumina MiSeq s...
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PREMISE OF THE STUDY Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed using Illumina sequencing for further genetic diversity studies of Stipa purpurea (Poaceae). METHODS AND RESULTS Twenty-nine polymorphic and eight monomorphic EST-SSR loci were developed and characterized in 90 individuals from nine S. purpurea populations. The number of alleles per locus range...
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PREMISE OF THE STUDY The first set of expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed and characterized for Speranskia tuberculata (Euphorbiaceae), a traditional medicinal plant endemic to northern China, to explore the effects of recent habitat fragmentation on the genetic diversity and structure of this species. METHODS AND RESULTS In this study, a total of 18...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2016
ISSN: 2168-0450
DOI: 10.3732/apps.1600113