Development and assessment of a rapid method to detect Escherichia coli O26, O111 and O157 in retail minced beef
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Development and assessment of a rapid method to detect Escherichia coli O26, O111 and O157 in retail minced beef.
A molecular-based detection method was developed to detect Escherichia coli O26, O111 and O157 in minced (ground) beef samples. This method consists of an initial overnight enrichment in modified tryptone soya broth (mTSB) and novobiocin prior to DNA extraction and subsequent serogrouping using a triplex PCR. This method has a low limit of detection and results are available within 24 hours of ...
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ژورنال
عنوان ژورنال: International Journal of Hygiene and Environmental Health
سال: 2007
ISSN: 1438-4639
DOI: 10.1016/j.ijheh.2006.09.004