Developing SyrinOX total antioxidant capacity assay for measuring antioxidants in humans
نویسندگان
چکیده
منابع مشابه
A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples
A new method for rapidly determining total antioxidant capacity (TAC) in a biological sample has been devised and evaluated. A surfactant present in the reagent causes the lysis of cells in the sample. Glutathione, ascorbic acid, protein thiols and other main antioxidant substances in the sample react with a specific redox chromogen (2,6-dichlorophenolindophenol) and produce a change in the col...
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Milk contains plenty of enzymatic and non-enzymatic antioxidant components that probably account for the vital antioxidant protection of the infants at early stages of life against the development of complications induced by oxygen free radicals. Indigenous milk enzymes play a key role in regulating lactogenesis, including active involution of mammary gland. Moreover, they are essential constit...
متن کاملEffect of hemodialysis on total antioxidant capacity and serum antioxidants in patients with chronic renal failure.
Cardiovascular disease is the major cause of mortality in patients receiving hemodialysis for chronic renal failure. Increased lipid peroxidation and depletion of chain-breaking antioxidants may contribute to increased risk of atherosclerosis. We have therefore assessed the effect of a single episode of hemodialysis on antioxidant status in 22 patients and control subjects. Overall, total antio...
متن کاملEfficient Assay for Total Antioxidant Capacity in Human Plasma Using a 96-Well Microplate
In the present study, we tried to establish an efficient assay for total antioxidant capacity (TAC) in human plasma using a 96-well microplate. TAC was assessed using lag time by antioxidants against the myoglobin-induced oxidation of 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) with hydrogen peroxide, and expressed as Trolox equivalent. The linearity of the calibration curve with...
متن کاملMeasurement of total antioxidant capacity.
The accurate assessment of oxidative stress in biological systems is a problem for all investigators working on the role of free radical damage in disease. Numerous assays have been described to measure various free radical damage products or antioxidant status, and the plethora of available techniques attests to the fact that no ideal method is available. The concept of a single test that migh...
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ژورنال
عنوان ژورنال: International Journal of Experimental Pathology
سال: 2012
ISSN: 0959-9673
DOI: 10.1111/iep.12001