Developing Microsatellite Multiplex PCR Panels for Topmouth Culter (Culter alburnus) and their Application in Parentage Assignment
نویسندگان
چکیده
منابع مشابه
Development of new polymorphic microsatellite markers in topmouth culter (Culter alburnus) and determination of their applicability in Culter mongolicus.
Fifteen new polymorphic microsatellite markers were developed for Culter alburnus. In 32 individuals representing a wild population of the Danjiangkou Reservoir, Hubei, China, the number of alleles at these loci varied between 2 and 10, with an average of 5.5. The average observed and expected heterozygosities were 0.664 and 0.681, respectively. The polymorphism information content of 11 loci w...
متن کاملIsolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky).
The topmouth culter (Culter alburnus) is an economically important freshwater fish, which is widely distributed throughout large rivers, reservoirs, and lake areas of China. We report here the isolation and characterization of 32 new polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA)12 and (GA)12 probes. The variability of these microsatellites was tested...
متن کاملOrganization and Variation Analysis of 5S rDNA in Different Ploidy-level Hybrids of Red Crucian Carp × Topmouth Culter
Through distant crossing, diploid, triploid and tetraploid hybrids of red crucian carp (Carassius auratus red var., RCC♀, Cyprininae, 2n = 100) × topmouth culter (Erythroculter ilishaeformis Bleeker, TC♂, Cultrinae, 2n = 48) were successfully produced. Diploid hybrids possessed 74 chromosomes with one set from RCC and one set from TC; triploid hybrids harbored 124 chromosomes with two sets from...
متن کاملDissecting the role of transforming growth factor-β1 in topmouth culter immunobiological activity: a fundamental functional analysis
Transforming growth factor-β1 (TGF-β1) has been proven to function primarily in mammalian immunobiological activity, but information regarding the immune role of TGF-β1 in teleosts is limited. In the present study, we describe the cDNA cloning and characterization of the TGF-β1 molecule in the topmouth culter. TGF-β1 is highly expressed in immune-related tissues of the culter, including the thy...
متن کاملDevelopment of multiplex microsatellite PCR panels for the seagrass Thalassia hemprichii (Hydrocharitaceae)1
UNLABELLED PREMISE OF THE STUDY New microsatellites were developed for the seagrass Thalassia hemprichii (Hydrocharitaceae), a long-lived seagrass species that is found throughout the shallow waters of tropical and subtropical Indo-West Pacific. Three multiplex PCR panels were designed utilizing new and previously developed markers, resulting in a toolkit for generating a 16-locus genotype. ...
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ژورنال
عنوان ژورنال: Pakistan Journal of Zoology
سال: 2021
ISSN: 0030-9923
DOI: 10.17582/journal.pjz/20180722120713