Determining the Topology of the Acid-Sensing Ion Channel Intracellular Domains
نویسندگان
چکیده
منابع مشابه
Acid-sensing ion channel 1a regulates the survival of nucleus pulposus cells in the acidic environment of degenerated intervertebral discs
Objective(s): Activation of acid-sensing ion channel 1a (ASIC1a) is responsible for tissue injury caused by acidosis in nervous systems. But its physiological and pathological roles in nucleus pulposus cells (NPCs) are unclear. The aim of this study is to investigate whether ASIC1a regulates the survival of NPCs in the acidic environment of degenerated discs. Materials and Methods: NPCs were i...
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Increased extracellular proton concentrations during neurotransmission are converted to excitatory sodium influx by acid-sensing ion channels (ASICs). 10-fold sodium/potassium selectivity in ASICs has long been attributed to a central constriction in the channel pore, but experimental verification is lacking due to the sensitivity of this structure to conventional manipulations. Here, we explor...
متن کاملStructural domains underlying the activation of acid-sensing ion channel 2a.
The acid-sensing ion channels (ASICs) are a family of ion channels expressed throughout the mammalian nervous system. The principal activator of ASICs is extracellular protons, and ASICs have been demonstrated to play a significant role in many physiologic and pathophysiologic processes, including synaptic transmission, nociception, and fear. However, not all ASICs are proton-sensitive: ASIC2a ...
متن کاملacid-sensing ion channel 1a regulates the survival of nucleus pulposus cells in the acidic environment of degenerated intervertebral discs
objective(s): activation of acid-sensing ion channel 1a (asic1a) is responsible for tissue injury caused by acidosis in nervous systems. but its physiological and pathological roles in nucleus pulposus cells (npcs) are unclear. the aim of this study is to investigate whether asic1a regulates the survival of npcs in the acidic environment of degenerated discs. materials and methods: npcs were is...
متن کاملA Nonproton Ligand Sensor in the Acid-Sensing Ion Channel
Acid-sensing ion channels (ASICs) have long been considered as extracellular proton (H(+))-gated cation channels, and peripheral ASIC3 channels seem to be a natural sensor of acidic pain. Here, we report the identification of a nonproton sensor on ASIC3. We show first that 2-guanidine-4-methylquinazoline (GMQ) causes persistent ASIC3 channel activation at the normal pH. Using GMQ as a probe and...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2021
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2020.11.2112