Determining the optimal pancreatic decellularization protocol, taking into account tissue morphological features

Introduction. Developing a tissue-engineered pancreatic construct (TEPC) involves search for matrices/scaffolds capable of mimicking the structure and composition natural extracellular matrix (ECM), which is an important component tissue microenvironment. A cell-free, tissue-specific obtained from pancreas decellularization seems to be most suitable creation TEPC. The choice protocol should take into account morphological characteristics original pancreas. Preservation architectonics native in decellularized (DPM), presence ECM components allow conditions prolonged vital activity functionally active islet (insulin-producing) cells when creating Objective : determine optimal parameters deceased donor with fibrosis, lipomatosis, without pronounced signs fibrosis lipomatosis. Materials methods . We used caudal part after multiorgan procurement donors, was unsuitable transplantation. Tissue-specific by combination physical chemical decellularization. freeze-thaw cycle two protocols using osmotic shock were used. Samples initial fragments subjected histological analysis. Result s. It shown that physico-chemical method cycles effective severe lipomatosis; shock, but different variants, diffuse Conclusion For complete human decellularization, correlated features tissue.