Determining protein stability in cell lysates by pulse proteolysis and Western blotting
نویسندگان
چکیده
منابع مشابه
Determining Biophysical Protein Stability in Lysates by a Fast Proteolysis Assay, FASTpp
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp). Combining unfolding by a temperature gradient in a thermal cycler with simultaneous proteolytic cleavage of the unfolded state, we probed stability of single domains in lysates. ...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2009
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.115