Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real-time RT-PCR
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چکیده
منابع مشابه
Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real-time RT-PCR.
Gene expression is differently regulated in every cell even though the cells are included in the same tissue. For this reason, we need to measure the amount of mRNAs in a single cell to understand transcription mechanism better. However, there are no accurate, rapid and appropriate methods to determine the exact copy numbers of particular mRNAs in a single cell. We therefore developed a procedu...
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ژورنال
عنوان ژورنال: Journal of Experimental Biology
سال: 2005
ISSN: 1477-9145,0022-0949
DOI: 10.1242/jeb.01625