Determination of scattering coefficient and scattering anisotropy factor of tissue-mimicking phantoms using line-field confocal optical coherence tomography (LC-OCT)

Line-field confocal optical coherence tomography (LC-OCT) is an imaging modality based on a combination of time-domain and reflectance microscopy. LC-OCT provides three-dimensional images semi-transparent samples with spatial resolution ∼ 1 μm. The technique primarily applied to in vivo skin imaging. image contrast arises from the backscattering incident light by sample microstructures, which determined scattering properties sample, characterized coefficient μs anisotropy factor g. In biological tissues, are organization, structure refractive indexes sample. measurement these using would therefore allow quantitative characterization tissues vivo. We present method for extracting two g tissue-mimicking phantoms 3D images. mean values over lateral field view 1.2 mm × 0.5 (x y). It can be monolayered bilayered samples, where it allows extraction each layer. Our approach calibration phantom known application theoretical model intensity depth profiles acquired LC-OCT. was experimentally tested against integrating spheres collimated transmission measurements set phantoms.