Determination of Protein-Ligand Interactions Using Accelerator Mass Spectrometry: Modified Crosslinking Assay
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چکیده
منابع مشابه
Retraction: Determination of protein-ligand interactions using accelerator mass spectrometry: modified crosslinking assay.
A highly sensitive detection method for the determination of protein-ligand interactions has been developed. Radiocarbon-labeled 17beta-estradiol was incubated with estrogen receptor-alpha; as a selective binding partner, and covalently attached using crosslinking agents, to form covalently linked protein-ligand complexes. After separation using a denaturing gel, the (14)C content in the sliced...
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A new electrospray ionization mass spectrometry (ES-MS) approach for quantifying protein-ligand complexes that are prone to in-source (gas-phase) dissociation is described. The method, referred to here as the reference ligand ES-MS method, is based on the direct ES-MS assay and competitive ligand binding. A reference ligand (L(ref)), which binds specifically to the protein (P), at the same bind...
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A new method that utilizes matrix-assisted laser desorption/ionization (MALDI) mass spectrometry and exploits the hydrogen/deuterium (H/D) exchange properties of proteins was developed for measuring the thermodynamic properties of protein-ligand complexes in solution. Dissociation constants (Kd values) determined by the method for five model protein-ligand complexes that included those with sma...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2009
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.25.731