Determination of Permethrin Resistance Allele Frequency of Human Head Louse Populations by Quantitative Sequencing
نویسندگان
چکیده
منابع مشابه
Permethrin Resistance Due to Knockdown Gene Mutations is Prevalent in Human Head Louse Populations
Permethrin resistance in head lice is mainly conferred by the knockdown resistance (kdr) trait, a voltagesensitive sodium channel (VSSC) insensitivity factor. Three VSSC mutations have been identified and confirmed to reduce the sensitivity of VSSC to permethrin. A step-wise resistance monitoring system has been established based on molecular resistance detection techniques. Quantitative sequen...
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The study examines the extent and frequency of a knockdown-type resistance allele (kdr type) in North American populations of human head lice. Lice were collected from 32 locations in Canada and the United States. DNA was extracted from individual lice and used to determine their zygosity using the serial invasive signal amplification technique to detect the kdr-type T917I (TI) mutation, which ...
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Quantitative determination of the allele frequency of single-nucleotide polymorphism (SNP) in pooled DNA samples is a promising approach to clarify the relationships between SNPs and diseases. Here, we present such a simple, accurate, and inexpensive method for quantitative determining the allele frequency in pooled DNA samples. Three steps of DNA pooling, PCR amplification and sequencing are i...
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Head lice, Pediculis capitis De Geer, populations were investigated for permethrin and malathion resistance after initial establishment of a discriminating dose of topical application bioassay with body lice, Pediculus humanus L. For both insecticides, approximately 2 times the lethal dose (LD)95 at 4 h was selected, 2 ng of permethrin and 100 ng of malathion per head louse, respectively. Head ...
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Genotyping-by-Sequencing (GBS) is an excellent tool for characterising genetic variation between plant genomes. To date, its use has been reported only for genotyping of single individuals. However, there are many applications where resolving allele frequencies within populations on a genome-wide scale would be very powerful, examples include the breeding of outbreeding species, varietal protec...
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ژورنال
عنوان ژورنال: Journal of Medical Entomology
سال: 2008
ISSN: 0022-2585,1938-2928
DOI: 10.1093/jmedent/45.5.912