Determination of keratin degradation by fungi using keratin azure

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Determination of keratin degradation by fungi using keratin azure.

Azure dye-impregnated sheep's wool keratin (keratin azure) was incorporated in a high pH medium and overlaid on a keratin-free basal medium. The release and diffusion of the azure dye into the lower layer indicated production of keratinase. Fifty-eight fungal taxa, including 49 members of the Arthrodermataceae, Gymnoascaceae and Onygenaceae (Order Onygenales), were assessed for keratin degradat...

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Keratinases and microbial degradation of Keratin

The present review deals with fungal keratinases including that of dermatophytes. Bacterial keratinases were also included. Temperature and substrate relationship keratinase production has also been discussed. Keratin degradation and industrial involvement of keratinase producing fungi is also reviewed.

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Keratin 13 is a more specific marker of conjunctival epithelium than keratin 19

Introduction To evaluate the expression patterns of cytokeratin (K) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether K13 could be used as a marker for conjunctival epithelium. Methods: Total RNA was isolated from the human conjunctiva and central cornea. Those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea wer...

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ژورنال

عنوان ژورنال: Medical Mycology

سال: 2004

ISSN: 1369-3786,1460-2709

DOI: 10.1080/13693780310001644680