Determinants of Helix-Loop-Helix Dimerization Affinity
نویسندگان
چکیده
منابع مشابه
ID helix-loop-helix proteins
The helix-loop-helix (HLH) family of transcription factors comprises >200 members, which have been identified in organisms from yeast to man (reviewed in Littlewood and Evan, 1995; Massari and Murre, 2000). In metazoa, HLH proteins function in the coordinate regulation of gene expression, orchestrating cell cycle control, cell lineage commitment and cell differentiation. An essential role has b...
متن کاملThe leucine zipper of TFE3 dictates helix-loop-helix dimerization specificity.
TFE3 is a DNA-binding protein that activates transcription through the muE3 site of the immunoglobulin heavy-chain enhancer. Its amino acid sequence reveals two putative protein dimerization motifs: a helix-loop-helix (HLH) and an adjacent leucine zipper. We show here that both of these motifs are necessary for TFE3 to homodimerize and to bind DNA in vitro. Using a dominant negative TFE3 mutant...
متن کاملId2 promotes apoptosis by a novel mechanism independent of dimerization to basic helix-loop-helix factors.
Members of the helix-loop-helix (HLH) family of Id proteins have demonstrated roles in the regulation of differentiation and cell proliferation. Id proteins inhibit differentiation by HLH-mediated heterodimerization with basic HLH transcription factors. This blocks their sequence-specific binding to DNA and activation of target genes that are often expressed in a tissue-specific manner. Id prot...
متن کاملExpression of basic helix-loop-helix proteins in the glomeruli.
BACKGROUND Basic helix loop helix (bHLH) proteins play a critical role in the differentiation of not only striated muscle cells but also adipocytes, neuron cells and smooth muscle cells. Previous studies have established in vitro mouse mesangial cells (MCs) to maintain the differentiated smooth muscle phenotype. MATERIALS AND METHODS The purpose of the present study was to clone bHLH proteins...
متن کاملHelix-loop-helix proteins: regulators of transcription in eucaryotic organisms.
The helix-loop-helix (HLH) family of transcriptional regulatory proteins are key players in a wide array of developmental processes. Over 240 HLH proteins have been identified to date in organisms ranging from the yeast Saccharomyces cerevisiae to humans (6). Studies in Xenopus laevis, Drosophila melanogaster, and mice have convincingly demonstrated that HLH proteins are intimately involved in ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.5.2683