Detection of Virulence Genes Stx1, Stx2, eae A and hly A in E.coli Isolated from Local Minced Meat

Abstract The current study included the investigation of E. coli bacteria in random samples ground meat, detection virulence genes isolates, and isolates resistance to some widely used antibiotics. Twenty-four minced meat were collected isolate identify by phenotypic, microscopic characteristics traditional biochemical tests done. Antibiotic susceptibility tests, genomes extracted, factors Stx1, Stx2, eae A hly investigated multiplex PCR technique. 7 showed Colonies with a metallic green luster on EMB medium, gram negative, rod shaped bacteria, facultative anaerobic positive for indole, methyl red catalase negative Fox Proscore, urease oxidase tests. All containing gene, 6 gene Stx1 , 5 4 Stx2 gene. results shown high all types antibiotics used, as possessed multiple antibiotic resistance.